Journal: Microbial cell factories

Article Title: Novel enterocin E20c purified from Enterococcus hirae 20c synergised with ß-lactams and ciprofloxacin against Salmonella enterica.

doi: 10.1186/s12934-020-01352-x

Figure Lengend Snippet: Fig. 1 Purification and characterization of E20c. a U-HPLC chromatogram of the purified E20c. b SDS-PAGE of purified E20c. Lane 1: molecular weight marker. Lane 2: cation-exchange fraction eluted with 0.4 mM NaCl-containing sodium acetate buffer. Lane 3: cation-exchange fraction eluted with 0.2 mM NaCl-containing sodium acetate buffer c PMF analysis of E20c. The PMF followed by MASCOT search showed the matched amino acid residues (in bold red) of the peptide fragments with the hypothetical protein of E. faecalis following NCBI BLASTsearch

Article Snippet: The active fractions obtained from Biogel PD-10 column were pooled and applied on SP-Sepharose Fast Flow cation-exchange column (50 × 10 mm; GE Healthcare) and eluted with a linear salt gradient of 0.1 to 1 M NaCl in sodium acetate buffer.

Techniques: Purification, SDS Page, Molecular Weight, Marker